根据基尼的评论

with open("your_file.txt", "r") as f:
    lines = f.readlines()
    new_lines = []
    idx_lines_wanted = [2,3,4]
    for i, line in enumerate(lines):
        if i > 4:
            break
        if i in idx_lines_wanted:
             new_lines.append(line)

with open("your_file.txt", "w") as f:
    for line in new_lines:
        f.write(line)

关于这个: 如果文件顶部有1-100行要删除

idx_lines_wanted = [x for x in range(0,101)]

if i > len(idx_lines_wanted) -1: